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The hub DEGs were validated in ischemic cardiomyopathy patients with end-stage heart failure <t>using</t> <t>single-cell</t> <t>sequencing</t> data. (A) The overall clustering of cells in ischemic cardiomyopathy patients with end-stage heart failure. (B) The hub genes were validated to be highly expressed in adipocytes in ischemic cardiomyopathy patients with end-stage heart failure, including STAT3, MDM2, LRP1, IRS2, PRKCD, CCND2 and CISH.
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snRNA‐Seq identifies distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.

Journal: Journal of Neurochemistry

Article Title: A High‐Resolution Transcriptomic Atlas of Cell Types in the Ventral Visual Thalamus

doi: 10.1111/jnc.70406

Figure Lengend Snippet: snRNA‐Seq identifies distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.

Article Snippet: Resource availability : A user‐friendly interface for visualizing and exploring the snRNA‐Seq data is available at the Broad Institute Single Cell Portal at https://singlecell.broadinstitute.org/single_cell/study/SCP3575 .

Techniques: Infection, Injection, Labeling, Transgenic Assay, Isolation, Expressing, Marker

snRNA‐Seq elucidates 13 retinorecipient neuronal types in vLGN. (A) UMAP (uniform manifold approximation and projection) of 4489 neuron cell nuclei from the ventral lateral geniculate nucleus, clustered by expression of high‐variance genes and colored according to cluster identity. (B) Neuronal UMAP recolored to illustrate expression of 89 mCherry+ vLGN nuclei following AAV injection in neurons. (C) Dot plot of neuronal clusters showing the expression of inhibitory marker genes ( Gad1 and Gad2 ) and excitatory marker genes ( Slc17a6 and Slc17a7 ) separated by original identity. (D) Dot plot of neuronal clusters showing the expression of Nxph1 (vLGNe) separated by original identity. (E) Neuron UMAP recolored to illustrate expression levels of Nxph1 .

Journal: Journal of Neurochemistry

Article Title: A High‐Resolution Transcriptomic Atlas of Cell Types in the Ventral Visual Thalamus

doi: 10.1111/jnc.70406

Figure Lengend Snippet: snRNA‐Seq elucidates 13 retinorecipient neuronal types in vLGN. (A) UMAP (uniform manifold approximation and projection) of 4489 neuron cell nuclei from the ventral lateral geniculate nucleus, clustered by expression of high‐variance genes and colored according to cluster identity. (B) Neuronal UMAP recolored to illustrate expression of 89 mCherry+ vLGN nuclei following AAV injection in neurons. (C) Dot plot of neuronal clusters showing the expression of inhibitory marker genes ( Gad1 and Gad2 ) and excitatory marker genes ( Slc17a6 and Slc17a7 ) separated by original identity. (D) Dot plot of neuronal clusters showing the expression of Nxph1 (vLGNe) separated by original identity. (E) Neuron UMAP recolored to illustrate expression levels of Nxph1 .

Article Snippet: Resource availability : A user‐friendly interface for visualizing and exploring the snRNA‐Seq data is available at the Broad Institute Single Cell Portal at https://singlecell.broadinstitute.org/single_cell/study/SCP3575 .

Techniques: Expressing, Injection, Marker

The hub DEGs were validated in ischemic cardiomyopathy patients with end-stage heart failure using single-cell sequencing data. (A) The overall clustering of cells in ischemic cardiomyopathy patients with end-stage heart failure. (B) The hub genes were validated to be highly expressed in adipocytes in ischemic cardiomyopathy patients with end-stage heart failure, including STAT3, MDM2, LRP1, IRS2, PRKCD, CCND2 and CISH.

Journal: Frontiers in Cardiovascular Medicine

Article Title: Senescence-related epicardial adipocyte genes lead to immune infiltration and myocardial infarction progression

doi: 10.3389/fcvm.2026.1759091

Figure Lengend Snippet: The hub DEGs were validated in ischemic cardiomyopathy patients with end-stage heart failure using single-cell sequencing data. (A) The overall clustering of cells in ischemic cardiomyopathy patients with end-stage heart failure. (B) The hub genes were validated to be highly expressed in adipocytes in ischemic cardiomyopathy patients with end-stage heart failure, including STAT3, MDM2, LRP1, IRS2, PRKCD, CCND2 and CISH.

Article Snippet: Single-cell sequencing data were obtained from the Single Cell Portal ( https://singlecell.broadinstitute.org/ ) to explore the hub gene expression in different cell lines (SCP1849).

Techniques: Single Cell, Sequencing